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dc.contributor.authorCaldeira, Dayene De Assis Fernandes
dc.contributor.authorMesquita, Flvia Muniz
dc.contributor.authorPinheiro, Felipe Gomes
dc.contributor.authorOliveira, Dahienne Ferreira
dc.contributor.authorSilva Oliveira, Luis Felipe
dc.contributor.authorNascimento, José Hamilton Matheus Do
dc.contributor.authorTakiya, Christina Maeda
dc.contributor.authorMaciel, Leonardo
dc.contributor.authorZin, Walter Araújo
dc.contributor.otherSilva Oliveira, Luis Felipe
dc.date.accessioned2021-01-22T14:56:21Z
dc.date.available2021-01-22T14:56:21Z
dc.date.issued2021
dc.identifier.citationCaldeira, D. D. A. F., Mesquita, F. M., Pinheiro, F. G., Oliveira, D. F., Oliveira, L. F. S., Nascimento, J. H. M., Takiya, C. M., Maciel, L. & Zin, W. A. (2021). Acute exposure to C60 fullerene damages pulmonary mitochondrial function and mechanics. Nanotoxicology, 15(3), 352-365. https://doi.org/10.1080/17435390.2020.1863498es_PE
dc.identifier.issn1743-5390
dc.identifier.urihttps://hdl.handle.net/20.500.12724/12361
dc.descriptionIndexado en Scopuses_PE
dc.description.abstractC60 fullerene (C60) nanoparticles, a nanomaterial widely used in technology, can offer risks to humans, overcome biological barriers, and deposit onto the lungs. However, data on its putative pulmonary burden are scanty. Recently, the C60 interaction with mitochondria has been described in vitro and in vivo. We hypothesized that C60 impairs lung mechanics and mitochondrial function. Thirty-five male BALB/c mice were randomly divided into two groups intratracheally instilled with vehicle (0.9% NaCl + 1% Tween 80, CTRL) or C60 (1.0 mg/kg, FUL). Twenty-four hours after exposure, 15 FUL and 8 CTRL mice were anesthetized, paralyzed, and mechanically ventilated for the determination of lung mechanics. After euthanasia, the lungs were removed en bloc at end-expiration for histological processing. Lung tissue elastance and viscance were augmented in FUL group. Increased inflammatory cell number, alveolar collapse, septal thickening, and pulmonary edema were detected. In other six FUL and six CTRL mice, mitochondria expressed reduction in state 1 respiration [FUL = 3.0 ± 1.14 vs. CTRL = 4.46 ± 0.9 (SEM) nmol O2/min/mg protein, p = 0.0210], ATP production (FUL = 122.6 ± 18 vs. CTRL = 154.5 ± 14 µmol/100 µg protein, p = 0.0340), and higher oxygen consumption in state 4 [FUL = 12.56 ± 0.9 vs. CTRL = 8.26 ± 0.6], generation of reactive oxygen species (FUL 733.1 ± 169.32 vs. CTRL = 486.39 ± 73.1 nmol/100 µg protein, p = 0.0313) and reason ROS/ATP [FUL = 8.73 ± 2.3 vs. CTRL = 2.99 ± 0.3]. In conclusion, exposure to fullerene C60 impaired pulmonary mechanics and mitochondrial function, increased ROS concentration, and decrease ATP production.es_PE
dc.formatapplication/pdfes_PE
dc.language.isoenges_PE
dc.publisherTaylor & Francises_PE
dc.relation.ispartofurn:issn:1743-5390
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.sourceRepositorio Institucional - Ulimaes_PE
dc.sourceUniversidad de Limaes_PE
dc.subjectNanotecnologíaes_PE
dc.subjectFullerenoes_PE
dc.subjectPulmoneses_PE
dc.subjectNanotechnologyes_PE
dc.subjectFullerenees_PE
dc.subjectLungses_PE
dc.subject.classificationPendiente / Pendientees_PE
dc.titleAcute exposure to C60 fullerene damages pulmonary mitochondrial function and mechanicses_PE
dc.typeinfo:eu-repo/semantics/article
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.type.otherArtículo en Scopuses_PE
ulima.areas.lineasdeinvestigacionCalidad de vida y bienestar / Saludes_PE
dc.identifier.journalNanotoxicologyes_PE
dc.publisher.countryGBes_PE
dc.description.peer-reviewRevisión por pareses_PE
dc.subject.ocdeIngeniería civiles_PE
dc.identifier.doihttps://doi.org/10.1080/17435390.2020.1863498
ulima.autor.afiliacionIngeniería Civil y Arquitectura, Universidad de Lima (Scopus)es_PE
ulima.autor.carreraNo figura en la lista del año 2020es_PE


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